Analytical Data
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Gene name
SOAT1
- Application
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Alternative Names
SOAT1; ACACT; ACACT1; ACAT; ACAT1; SOAT; STAT; Sterol O-acyltransferase 1; Acyl-coenzyme A:cholesterol acyltransferase 1; ACAT-1; Cholesterol acyltransferase 1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35610
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Expression Region
249-321 aa
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AA Sequence
PPASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWG
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Molecular Weight
33.77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SOAT1 (Sterol O-acyltransferase 1) is an enzyme involved in the esterification of cholesterol, playing a crucial role in lipid metabolism and cellular homeostasis. Research on SOAT1 has gained momentum due to its significant implications in various pathological conditions, including atherosclerosis, obesity, and neurodegenerative diseases. Dysregulation of cholesterol metabolism is closely associated with these conditions, making SOAT1 a potential therapeutic target. The enzyme facilitates the conversion of free cholesterol into cholesteryl esters, which are stored in lipid droplets or used for membrane synthesis. Given the rising prevalence of metabolic disorders, understanding the structure and function of SOAT1 is essential for developing novel pharmacological interventions. Recent studies have focused on elucidating the molecular mechanisms governing SOAT1 activity and regulation, as well as its interactions with other metabolic pathways. Additionally, the recombinant production of SOAT1 provides an invaluable tool for functional assays and structural studies, leading to insights into its enzymatic properties and potential inhibitors. These advancements could pave the way for innovative therapeutic strategies aimed at mitigating cholesterol-related diseases. Overall, the exploration of SOAT1 and its recombinant protein forms reflects a critical intersection of biochemistry, pharmacology, and disease research, highlighting its importance in the quest for targeted treatments in lipid metabolism disorders.
