Analytical Data
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Gene name
ND1
- Application
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Alternative Names
ND1；MTND1；NADH1；ND1；NADH-ubiquinone oxidoreductase chain 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P03886
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Expression Region
1-318aa
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AA Sequence
MPMANLLLLIVPILIAMAFLMLTERKILGYMQLRKGPNVVGPYGLLQPFADAMKLFTKEPLKPATSTITLYITAPTLALTIALLLWTPLPMPNPLVNLNLGLLFILATSSLAVYSILWSGWASNSNYALIGALRAVAQTISYEVTLAIILLSTLLMSGSFNLSTLITTQEHLWLLLPSWPLAMMWFISTLAETNRTPFDLAEGESELVSGFNIEYAAGPFALFFMAEYTNIIMMNTLTTTIFLGTTYDALSPELYTTYFVTKTLLLTSLFLWIRTAYPRFRYDQLMHLLWKNFLPLTLALLMWYVSMPITISSIPPQT
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ND1 recombinant protein research focuses on the role of the ND1 gene, which encodes the NADH dehydrogenase subunit 1, a critical component of the mitochondrial respiratory chain complex I. This protein is essential for energy production in eukaryotic cells, facilitating the transfer of electrons from NADH to coenzyme Q in the mitochondrial membrane. Mutations or deficiencies in the ND1 gene have been associated with various mitochondrial diseases, leading to significant clinical manifestations, including neurodegenerative disorders and metabolic syndromes. The recombinant production of ND1 protein allows researchers to investigate its structure, function, and interactions with other mitochondrial components in detail. Moreover, such studies can aid in understanding the molecular mechanisms underlying mitochondrial dysfunction and the pathogenesis of related diseases. By developing therapeutic strategies that target ND1 or compensate for its dysfunction, researchers aim to provide novel treatments for patients affected by mitochondrial disorders. Thus, the exploration of ND1 recombinant protein serves as a pivotal area of research in both basic and clinical sciences, with implications for advancing our knowledge of mitochondrial biology and developing targeted therapeutic interventions.
