Analytical Data
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Gene name
ND5
- Application
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Alternative Names
ND5；MTND5；NADH5；ND5；NADH-ubiquinone oxidoreductase chain 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P03915
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Expression Region
1-603aa
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AA Sequence
MTMHTTMTTLTLTSLIPPILTTLVNPNKKNSYPHYVKSIVASTFIISLFPTTMFMCLDQEVIISNWHWATTQTTQLSLSFKLDYFSMMFIPVALFVTWSIMEFSLWYMNSDPNINQFFKYLLIFLITMLILVTANNLFQLFIGWEGVGIMSFLLISWWYARADANTAAIQAILYNRIGDIGFILALAWFILHSNSWDPQQMALLNANPSLTPLLGLLLAAAGKSAQLGLHPWLPSAMEGPTPVSALLHSSTMVVAGIFLLIRFHPLAENSPLIQTLTLCLGAITTLFAAVCALTQNDIKKIVAFSTSSQLGLMMVTIGINQPHLAFLHICTHAFFKAMLFMCSGSIIHNLNNEQDIRKMGGLLKTMPLTSTSLTIGSLALAGMPFLTGFYSKDHIIETANMSYTNAWALSITLIATSLTSAYSTRMILLTLTGQPRFPTLTNINENNPTLLNPIKRLAAGSLFAGFLITNNISPASPFQTTIPLYLKLTALAVTFLGLLTALDLNYLTNKLKMKSPLCTFYFSNMLGFYPSITHRTIPYLGLLTSQNLPLLLLDLTWLEKLLPKTISQHQISTSIITSTQKGMIKLYFLSFFFPLILTLLLIT
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ND5, a key subunit of the mitochondrial NADH dehydrogenase complex I, plays a crucial role in the electron transport chain, facilitating ATP production in eukaryotic cells. Its importance in cellular metabolism and energy homeostasis makes it a significant focus of research, particularly concerning mitochondrial diseases and metabolic disorders. Mutations in the ND5 gene have been linked to various pathologies, including Leber's hereditary optic neuropathy and other mitochondrial myopathies, underscoring the necessity for detailed studies on this protein. Recent advancements in molecular biology techniques, such as recombinant DNA technology and protein expression systems, have enabled researchers to produce ND5 in vitro, allowing for comprehensive functional analyses. Understanding the structural and functional dynamics of ND5 through recombinant protein studies can provide insights into its role in respiratory chain disorders, paving the way for potential therapeutic interventions. Furthermore, investigating the interactions between ND5 and other mitochondrial proteins may reveal mechanisms of regulation and signaling that are fundamental to mitochondrial function. Overall, ND5 recombinant protein research holds promise for elucidating the complexities of mitochondrial biology and addressing the challenges posed by related diseases.
