Analytical Data
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Gene name
SMARCD1
- Application
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Alternative Names
60 kDa BRG-1/Brm-associated factor subunit A; 60 kDa BRG1/Brm associated factor subunit A; BAF60A; BRG1 associated factor 60A; BRG1-associated factor 60A; Chromatin remodeling complex BAF60A subunit; CRACD1; Mammalian chromatin remodeling complex BRG1 associated factor 60A; Rsc6p; SMARCD1; SMRD1; SMRD1_HUMAN; SWI/SNF complex 60 kDa subunit A; SWI/SNF complex 60 kDa subunit; SWI/SNF related matrix associated actin dependent regulator of chromatin d1; SWI/SNF related. matrix associated. actin dependent regulator of chromatin. subfamily d. member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1; Swp73 like protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96GM5
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Expression Region
1-515 aa
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AA Sequence
MRAGFQSV APSGGAGASG GAGLGP GGTPGPPVRM GPAPGQGLYR SPMPGYPR PGMLPGSRMT PQGPSMGPPG YGGNPSVRPG LAQSGMDQSR KRPAPQQIQQ VQQQAVQNRN HNAKKKKMAD KILPQRIREL VPESQAYMDL LAFERKLDQT IMRKRLDIQE ALKRPIKQKR KLRIFISNTF NPAKSDAEDG EGTVASWELR VEGRLLEDSA LSKYDATKQK RKFSSFFKSL VIELDKDLYG PDNHLVEWHR TATTQETDGF QVKRPGDVNV RCTVLLMLDY QPPQFKLDPR LARLLGIHTQ TRPVIIQALW QYIKTHKLQD PHEREFVICD KYLQQIFESQ RMKFSEIPQR LHALLMPPEP IIINHVISVD PNDQKKTACY DIDVEVDDTL KTQMNSFLLS TASQQEIATL DNKIHETIET INQLKTQREF MLSFARDPQG FINDWLQSQC RDLKTMTDVV GNPEEERRAE FYFQPWAQEA VCRYFYSKVQ QRRQELEQAL GIRNT
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Molecular Weight
58.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMARCD1, also known as BAF60a, is a pivotal component of the SWI/SNF chromatin remodeling complex, which plays a critical role in regulating gene expression, cellular differentiation, and development. The SWI/SNF complex functions by altering the structure of chromatin, thereby facilitating or inhibiting the access of transcriptional machinery to DNA. Understanding SMARCD1's role has gained prominence due to its implications in various biological processes and diseases, including cancer, where mutations and dysregulations in chromatin remodeling factors have been linked to tumorigenesis. Recent studies have highlighted the importance of SMARCD1 in influencing cellular responses to external stimuli, regulating stem cell pluripotency, and modulating epigenetic landscapes. Researchers are focusing on characterizing the structural and functional properties of recombinant SMARCD1 to elucidate its specific interactions within the SWI/SNF complex and its downstream effects on gene regulation. This research is vital for developing targeted therapies that could manipulate chromatin dynamics in disease contexts, offering potential avenues for innovative treatment strategies. Understanding the intricate mechanisms mediated by SMARCD1 may ultimately contribute to advancements in regenerative medicine, cancer treatment, and the comprehension of developmental disorders.
