Analytical Data
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基因名
PNKP
- Application
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别名
PNKP；Bifunctional polynucleotide phosphatase/kinase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96T60
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表达区间
1-521aa
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氨基酸序列
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQ VELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLH PLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENL EKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIP RKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVL VATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPG RKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSG PLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLG SWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLF TATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILE IPFRLWVEPRLGRLYCQFSEG
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分子量
84 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNKP (Polynucleotide Kinase 3'-Phosphatase) is a crucial enzyme involved in DNA repair and RNA metabolism, playing a significant role in maintaining genomic stability. Mutations in the PNKP gene are associated with several neurological disorders, including Aicardi-Goutières syndrome and microcephaly, highlighting its importance in neurodevelopmental processes. Research into PNKP recombinant proteins has become increasingly significant as scientists aim to understand the enzyme’s structural and functional properties. By utilizing heterologous expression systems, researchers can produce purified PNKP proteins for in-depth studies, enabling them to analyze the enzyme’s activity, stability, and interactions with other biomolecules. Such investigations are essential for deciphering the molecular mechanisms behind PNKP's role in the DNA damage response and cellular reprogramming. Additionally, characterizing PNKP can offer insights into potential therapeutic strategies for conditions linked to its dysfunction, paving the way for new approaches in treating neurodevelopmental disorders and age-related diseases. The ongoing research is opening avenues for innovative treatments that could mitigate the consequences of PNKP-related pathologies, ultimately contributing to advancements in gene therapy and regenerative medicine.
