Cat: PA1000-7700

Recombinant Human COL10 Protein,His

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Analytical Data

  • Gene name

    COL10

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    COL10;CLL1;Collectin-10

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9LUA9

  • Expression Region

    1-373aa

  • AA Sequence

    MGYMCDFCGE QRSMVYCRSD AACLCLSCDR NVHSANALSK RHSRTLVCER CNAQPASVRC SDERVSLCQN CDWSGHDGKN STTTSHHKRQ TINCYSGCPS SAELSSIWSF CMDLNISSAE ESACEQGMGL MTIDEDGTGE KSGVQKINVE QPETSSAAQG MDHSSVPENS SMAKELGVCE DDFNGNLISD EVDLALENYE ELFGSAFNSS RYLFEHGGIG SLFEKDEAHE GSMQQPALSN NASADSFMTC RTEPIICYSS KPAHSNISFS GITGESNAGD FQDCGASSMK QLSREPQPWC HPTAQDIIAS SHATTRNNAV MRYKEKKKAR KFDKRVRYVS RKERADVRRR VKGRFVKSGE AYDYDPMSPT RSY

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

COL10, or type X collagen, is a fibrillar collagen primarily expressed in hypertrophic cartilage during endochondral ossification. It plays a crucial role in the development of long bones and is associated with the growth plate's maturation and subsequent mineralization. Research into COL10 recombinant proteins has gained traction due to their potential applications in regenerative medicine and tissue engineering, particularly for cartilage repair and osteoarthritis treatment. Understanding the structure and function of COL10 is vital for developing therapeutic strategies aimed at enhancing cartilage regeneration and understanding pathologies related to skeletal disorders. Recent advances in molecular biology and protein engineering have enabled the production of recombinant COL10 proteins, providing valuable tools for studying its role in cartilage biology and facilitating the development of novel biomaterials that mimic the native extracellular matrix. Furthermore, the ability to produce and modify COL10 in vitro opens new avenues for investigating cartilage-specific signaling pathways, interactions with other extracellular matrix components, and their influence on chondrocyte behavior, ultimately advancing our knowledge in orthopedics and regenerative therapies.

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