Analytical Data
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Gene name
CSTF3
- Application
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Alternative Names
CSTF3Cleavage stimulation factor subunit 3; CF-1 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit; CSTF 77 kDa subunit; CstF-77
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12996
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Expression Region
2-717aa
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AA Sequence
SGDGATEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA RKTYERLVAQ FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL WKCYLSYVRE TKGKLPSYKE KMAQAYDFAL DKIGMEIMSY QIWVDYINFL KGVEAVGSYA ENQRITAVRR VYQRGCVNPM INIEQLWRDY NKYEEGINIH LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ NTPQEAQQVD MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE ESRMKYEKVH SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK AREDTRTRHH VYVTAALMEY YCSKDKSVAF KIFELGLKKY GDIPEYVLAY IDYLSHLNED NNTRVLFERV LTSGSLPPEK SGEIWARFLA FESNIGDLAS ILKVEKRRFT AFKEEYEGKE TALLVDRYKF MDLYPCSASE LKALGYKDVS RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN TVEEAVRIIT GGAPELAVEG NGPVESNAVL TKAVKRPNED SDEDEEKGAV VPPVHDIYRA RQQKRIR
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Molecular Weight
82.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CSTF3 (Cleavage Stimulation Factor 3) is a critical protein involved in the process of mRNA 3' end processing, which is essential for the maturation of messenger RNA transcripts in eukaryotic cells. It plays a key role in the cleavage and polyadenylation of pre-mRNA, a vital step in gene expression regulation. Dysfunction or mutations in CSTF3 have been associated with various diseases, including cancer and genetic disorders, highlighting its importance in cellular function and development. Recent research has focused on the structure and function of CSTF3, as understanding its role in RNA metabolism can provide insights into the mechanisms underlying gene regulation and identify potential therapeutic targets. The recombinant protein form of CSTF3 allows for in-depth studies of its biochemical properties and interactions with other components of the RNA processing machinery. This research is paving the way for novel biotechnological applications and therapeutic interventions aimed at correcting or mitigating diseases linked to mRNA processing defects.
