Analytical Data
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基因名
BAS1
- Application
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别名
BAS1；2-Cys peroxiredoxin BAS1. chloroplastic
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96291
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表达区间
66-266aa
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氨基酸序列
KAQADDLPLVGNKAPDFEAEAVFDQEFIKVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRHSEFEKLNTEVLGVSVDSVFSHLAWVQTDRKSGGLGDLNYPLISDVTKSISKSFGVLIHDQGIALRGLFIIDKEGVIQHSTINNLGIGRSVDETMRTLQALQYIQENPDEVCPAGWKPGEKSMKPDPKLSKEYFSAI
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分子量
24.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BAS1, a gene encoding a branch chain aminotransferase, plays a crucial role in plant metabolism by regulating the synthesis of branched-chain amino acids (BCAAs) such as leucine, isoleucine, and valine. These amino acids are essential not only for protein synthesis but also for various metabolic processes, including energy production and stress response. The study of BAS1 recombinant proteins has gained traction in the field of plant biotechnology and metabolic engineering, as manipulating its expression can enhance stress tolerance, improve yield, and alter nutritional profiles in crops. Additionally, BAS1 has drawn interest due to its potential applications in synthetic biology, where engineered pathways can optimize BCAA production for use in pharmaceuticals, food, and feed industries. Research in this area often involves the cloning of the BAS1 gene, expression in suitable host systems, and subsequent characterization of the recombinant protein's enzymatic activity and physiological effects on plant growth and development. Understanding the molecular mechanisms underlying BAS1 function can provide valuable insights into plant resilience under abiotic stresses and contribute to sustainable agricultural practices in the face of climate change and food security challenges.
