Analytical Data
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Gene name
ND75
- Application
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Alternative Names
ND75；ND75；NADH-ubiquinone oxidoreductase 75 kDa subunit. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q94511
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Expression Region
28-315aa
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AA Sequence
SAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR
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Molecular Weight
47.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ND75 recombinant protein is derived from the ND75 gene, which encodes a multi-subunit complex of the oxidative phosphorylation pathway, playing a crucial role in cellular energy metabolism. The interest in ND75 has surged due to its significant involvement in mitochondrial function and ATP synthesis. Alterations or mutations within this gene can lead to various mitochondrial diseases, characterized by energy deficiency and a spectrum of clinical manifestations ranging from muscle weakness to neurological disorders. Research into the ND75 recombinant protein focuses on understanding its structural and functional properties, which can provide insights into mitochondrial pathophysiology. By producing ND75 through recombinant DNA technology, scientists aim to elucidate its role in energy production and its interaction with other mitochondrial components. This research not only enhances our understanding of metabolic diseases but also opens avenues for therapeutic interventions, including potential gene therapy or targeted treatments for individuals with mitochondrial dysfunction. The development of ND75 recombinant protein has implications for both basic research and clinical applications, paving the way for novel strategies to combat the challenges posed by mitochondrial disorders.
