Analytical Data
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Gene name
OXCT1
- Application
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Alternative Names
OXCT1；OXCT；SCOT；Succinyl-CoA:3-ketoacid coenzyme A transferase 1. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55809
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Expression Region
40-520aa
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AA Sequence
TKFYTDPVEAVKDIPDGATVLVGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN
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Molecular Weight
56.1kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
OXCT1, or 3-oxoacid CoA transferase 1, is an important enzyme involved in the metabolism of ketone bodies, particularly in the utilization of acetoacetate and its conversion into acetyl-CoA, which is crucial for energy production in various tissues, including the brain and heart. Recent studies have highlighted the significance of OXCT1 in metabolic health, as it plays a pivotal role in ketone body utilization during periods of fasting or low carbohydrate intake. Alterations in OXCT1 expression or activity have been linked to metabolic disorders such as obesity, diabetes, and neurological diseases, underscoring its potential as a therapeutic target. Research on recombinant OXCT1 protein aims to elucidate its biochemical properties, regulatory mechanisms, and interactions with other metabolic pathways. By producing this protein in a controlled laboratory setting, scientists can investigate its enzymatic activity, structural characteristics, and influence on cellular metabolism. This research not only enhances our understanding of OXCT1's role in energy homeostasis but also paves the way for developing strategies to modulate its function for therapeutic purposes, potentially offering new avenues for treating metabolic diseases associated with dysregulated ketone metabolism.
