Analytical Data
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Gene name
KRT77
- Application
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Alternative Names
KRT77；KRT1B；Keratin. type II cytoskeletal 1b
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7Z794
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Expression Region
1-578aa
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AA Sequence
MSHQFSSQSAFSSMSRRVYSTSSSAGSGGGSPAVGSVCYARGRCGGGGYGIHGRGFGSRSLYNLGGSRSISINLMGRSTSGFCQGGGVGGFGGGRGFGVGSTGAGGFGGGGFGGAGFGTSNFGLGGFGPYCPPGGIQEVTINQSLLEPLHLEVDPEIQRIKTQEREQIMVLNNKFASFIDKVRFLEQQNQVLQTKWELLQQVNTSTGTNNLEPLLENYIGDLRRQVDLLSAEQMRQNAEVRSMQDVVEDYKSKYEDEINKRTGSENDFVVLKKDVDAAYVSKVDLESRVDTLTGEVNFLKYLFLTELSQVQTHISDTNVILSMDNNRSLDLDSIIDAVRTQYELIAQRSKDEAEALYQTKYQELQITAGRHGDDLKNSKMEIAELNRTVQRLQAEISNVKKQIEQMQSLISDAEERGEQALQDAWQKLQDLEEALQQSKEELARLLRDYQAMLGVKLSLDVEIATYRQLLEGEESRMSGELQSHVSISVQNSQVSVNGGAGGGGSYGSGGYGGGSGGGYGGGRSYRGGGARGRSGGGYGSGCGGGGGSYGGSGRSGRGSSRVQIIQTSTNTSHRRILE
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Molecular Weight
63.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KRT77, a member of the keratin family, is a type of intermediate filament protein primarily expressed in specific epithelial tissues. Research into KRT77 has gained attention due to its potential roles in skin biology, particularly in the differentiation and structural integrity of keratinocytes. The keratin family is known for its diverse and crucial functions, including providing mechanical support and protection to epithelial cells. Abnormalities in keratin expression can contribute to various skin disorders and diseases, such as epidermolysis bullosa and certain forms of skin cancer. Understanding the molecular characteristics and expression patterns of KRT77 could offer insights into its functional roles in skin development and regeneration. Recent studies have focused on the gene expression regulation of KRT77, exploring its interactions with other proteins in the keratin network and identifying potential signaling pathways that might influence its function. The recombinant production of KRT77 protein allows for in-depth functional studies, aiding in the understanding of its potential as a biomarker for skin-related conditions and its role in therapeutic applications, including wound healing and skin regeneration. Overall, the exploration of KRT77 not only contributes to fundamental biological knowledge but also paves the way for novel clinical strategies in managing skin diseases.
