Analytical Data
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基因名
ANPEP
- Application
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别名
ANPEP；APN；CD13；PEPN；Aminopeptidase N
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P15144
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表达区间
69-967aa
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氨基酸序列
KAWNRYRLPN TLKPDSYRVT LRPYLTPNDR GLYVFKGSST VRFTCKEATD VIIIHSKKLN YTLSQGHRVV LRGVGGSQPP DIDKTELVEP TEYLVVHLKG SLVKDSQYEM DSEFEGELAD DLAGFYRSEY MEGNVRKVVA TTQMQAADAR KSFPCFDEPA MKAEFNITLI HPKDLTALSN MLPKGPSTPL PEDPNWNVTE FHTTPKMSTY LLAFIVSEFD YVEKQASNGV LIRIWARPSA IAAGHGDYAL NVTGPILNFF AGHYDTPYPL PKSDQIGLPD FNAGAMENWG LVTYRENSLL FDPLSSSSSN KERVVTVIAH ELAHQWFGNL VTIEWWNDLW LNEGFASYVE YLGADYAEPT WNLKDLMVLN DVYRVMAVDA LASSHPLSTP ASEINTPAQI SELFDAISYS KGASVLRMLS SFLSEDVFKQ GLASYLHTFA YQNTIYLNLW DHLQEAVNNR SIQLPTTVRD IMNRWTLQMG FPVITVDTST GTLSQEHFLL DPDSNVTRPS EFNYVWIVPI TSIRDGRQQQ DYWLIDVRAQ NDLFSTSGNE WVLLNLNVTG YYRVNYDEEN WRKIQTQLQR DHSAIPVINR AQIINDAFNL ASAHKVPVTL ALNNTLFLIE ERQYMPWEAA LSSLSYFKLM FDRSEVYGPM KNYLKKQVTP LFIHFRNNTN NWREIPENLM DQYSEVNAIS TACSNGVPEC EEMVSGLFKQ WMENPNNNPI HPNLRSTVYC NAIAQGGEEE WDFAWEQFRN ATLVNEADKL RAALACSKEL WILNRYLSYT LNPDLIRKQD ATSTIISITN NVIGQGLVWD FVQSNWKKLF NDYGGGSFSF SNLIQAVTRR FSTEYELQQL EQFKKDNEET GFGSGTRALE QALEKTKANI KWVKENKEVV LQWFTENSK
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分子量
104 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ANPEP, also known as aminopeptidase N, is a type II membrane-bound metallopeptidase that plays a crucial role in various physiological processes, including inflammation, immune response, and the metabolism of bioactive peptides. Its expression is particularly prevalent in the kidney, intestine, and immune cells, where it is involved in the regulation of peptide hormone activity and nutrient absorption. Research over the years has illuminated its significance in diseases such as hypertension, diabetes, and certain cancers, making it a potential therapeutic target. The study of recombinant ANPEP proteins has gained traction as scientists aim to elucidate its structure-function relationships and enzymatic mechanisms, as well as to identify its potential role as a biomarker. By using recombinant DNA technology, researchers can produce ANPEP in sufficient quantities, enabling detailed biochemical characterization, identification of substrate specificity, and investigating its interactions with inhibitors or modulators. This foundational research not only enhances our understanding of ANPEP's biological functions but also paves the way for developing novel therapeutic strategies aimed at modulating its activity in disease contexts, thus underscoring the protein's relevance in both basic and translational biomedical research.
