Analytical Data
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基因名
IDO
- Application
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别名
IDO；IDO；INDO；；Indoleamine 2.3-dioxygenase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P14902
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表达区间
1-403aa
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氨基酸序列
MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG
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分子量
49.3kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research background of IDO (indoleamine 2,3-dioxygenase) as a recombinant protein is rooted in its crucial role in immune regulation and tumor biology. IDO is an enzyme that catalyzes the degradation of tryptophan into kynurenine, an important pathway that modulates immune responses, particularly in the context of cancer and chronic infections. Elevated IDO activity has been associated with immune tolerance, allowing tumors to evade immune surveillance, thereby promoting tumor growth and metastasis. Understanding the mechanisms by which IDO contributes to immunosuppression has spurred interest in developing IDO inhibitors as novel therapeutic agents. Moreover, IDO's involvement in autoimmune diseases and neurodegenerative disorders has further broadened the scope of research surrounding this enzyme. The recombinant production of IDO proteins enables researchers to elucidate its structure-function relationship, assess its activity, and investigate potential inhibitors. Utilizing recombinant techniques also allows for the generation of modified versions of IDO that can be used in therapeutic applications or as biomarkers for disease progression. Overall, the study of IDO as a recombinant protein is significant for advancing our understanding of its biological roles and potential applications in immunotherapy and disease treatment.
