Analytical Data
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Gene name
SF3A3
- Application
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Alternative Names
SF3A3; SAP61; Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12874
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Expression Region
1-501 aa
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AA Sequence
METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLKQ IKEFHRKHPN EICVPMSVEF EELLKARENP SEEAQNLVEF TDEEGYGRYL DLHDCYLKYI NLKASEKLDY ITYLSIFDQL FDIPKERKNA EYKRYLEMLL EYLQDYTDRV KPLQDQNELF GKIQAEFEKK WENGTFPGWP KETSSALTHA GAHLDLSAFS SWEELASLGL DRLKSALLAL GLKCGGTLEE RAQRLFSTKG KSLESLDTSL FAKNPKSKGT KRDTERNKDI AFLEAQIYEY VEILGEQRHL THENVQRKQA RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK PIPYWLYKLH GLNINYNCEI CGNYTYRGPK AFQRHFAEWR HAHGMRCLGI PNTAHFANVT QIEDAVSLWA KLKLQKASER WQPDTEEEYE DSSGNVVNKK TYEDLKRQGL L
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Molecular Weight
58.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SF3A3, a key component of the spliceosomal complex, plays a crucial role in pre-mRNA splicing, a fundamental process in gene expression that produces mature mRNA by removing introns from pre-mRNA transcripts. Mutations and dysregulation of SF3A3 have been implicated in various cancers and genetic disorders, making it an important target for understanding the molecular mechanisms underlying these diseases. The study of recombinant SF3A3 protein is critical for elucidating its structural and functional properties, as well as its interactions with other spliceosomal components. By producing purified SF3A3 in a recombinant system, researchers can perform detailed biochemical assays and structural analyses, which will enhance our understanding of its role in splicing regulation and may provide insights into therapeutic strategies targeting spliceosomal machinery in disease contexts. Additionally, understanding SF3A3's functions and interactions could lead to the discovery of novel biomarkers for diagnosing and predicting outcomes in cancers associated with splicing anomalies. Overall, SF3A3 represents a significant focus in the field of molecular biology and cancer research, underlining the importance of developing and characterizing this recombinant protein for future studies.
