Analytical Data
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Gene name
tetM
- Application
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Alternative Names
tetM；tet；Tetracycline resistance Protein TetM
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q53770
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Expression Region
1-242aa
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AA Sequence
MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDFVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
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Molecular Weight
32.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TetM is a crucial protein involved in antibiotic resistance, specifically conferring resistance to tetracycline antibiotics in bacteria. This protein is classified as a ribosome protection protein, which enables bacteria to evade the inhibitory effects of tetracyclines by displacing the antibiotic from its binding site on the ribosome. The prevalence of tetM among various bacterial species, especially in pathogenic strains, has become a significant concern in public health due to the rising rates of antibiotic resistance. Researchers have focused on studying the molecular mechanisms of tetM to understand its structure and function better, which may provide insights into the development of novel antimicrobial strategies. Additionally, the transfer of tetM genes among bacteria highlights the urgent need for surveillance and effective measures to combat the spread of antibiotic resistance. Investigating the recombinant tetM protein can offer opportunities to identify potential drug targets and devise new therapeutic approaches to tackle infections caused by tetracycline-resistant bacteria, ultimately contributing to improved treatment regimens and health outcomes.
