Analytical Data
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基因名
CHST12
- Application
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别名
CHST12; UNQ500/PRO1017Carbohydrate sulfotransferase 12; EC 2.8.2.5; Chondroitin 4-O-sulfotransferase 2; Chondroitin 4-sulfotransferase 2; C4ST-2; C4ST2; Sulfotransferase Hlo
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRB3
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表达区间
1-414aa
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氨基酸序列
MTKARLFRLWLVLGSVFMILLIIVYWDSAGAAHFYLHTSFSRPHTGPPLPTPGPDRDRELTADSDVDEFLDKFLSAGVKQSDLPRKETEQPPAPGSMEESVRGYDWSPRDARRSPDQGRQQAERRSVLRGFCANSSLAFPTKERAFDDIPNSELSHLIVDDRHGAIYCYVPKVACTNWKRVMIVLSGSLLHRGAPYRDPLRIPREHVHNASAHLTFNKFWRRYGKLSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFAVPMLRLYANHTSLPASAREAFRAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLCHPCQIDYDFVGKLETLDEDAAQLLQLLQVDRQLRFPPSYRNRTASSWEEDWFAKIPLAWRQQLYKLYEADFVLFGYPKPENLLRD
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分子量
71.28 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHST12 (Carbohydrate Sulfotransferase 12) is a member of the sulfotransferase enzyme family, which plays a crucial role in the biosynthesis of sulfated glycosaminoglycans (GAGs) such as heparan sulfate and chondroitin sulfate. These molecules are vital for numerous biological processes, including cell signaling, proliferation, and development. Abnormalities in GAG sulfation patterns have been implicated in various diseases, including cancer, neurological disorders, and cardiovascular diseases. Consequently, understanding the enzymatic mechanisms and regulation of CHST12 is of significant interest. Researchers focus on characterizing recombinant CHST12 to explore its substrate specificity, catalytic activity, and overall functioning within the cellular context. By studying CHST12 in vitro through recombinant protein expression, scientists aim to elucidate its role in GAG modification and potential implications for therapeutic interventions. The successful production and analysis of recombinant CHST12 also pave the way for developing novel biomolecular tools and enhancing our understanding of sulfation pathways, which could lead to breakthroughs in disease treatment and regenerative medicine.
