Analytical Data
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Gene name
aro-2
- Application
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Alternative Names
aro-2；TPH；TPRH；TRPH；Tryptophan 5-hydroxylase 1
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12640
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Expression Region
1-432aa
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AA Sequence
MSTFGHYFRVTTYGESHCKSVGCIVDGVPPGMELTEDDIQPQMTRRRPGQSAITTPRDEKDRVIIQSGTEFGVTLGTPIGMLVMNEDQRPKDYGNKTMDIYPRPSHADWTYLEKYGVKASSGGGRSSARETIGRVAAGAIAEKYLKLAYGVEIVAFVSSVGSEHLFPPTAEHPSPSTNPEFLKLVNSITRETVDSFLPVRCPDAEANKRMEDLITKFRDNHDSIGGTVTCVIRNVPSGLGEPAFDKLEAMLAHAMLSIPATKGFEVGSGFGGCEVPGSIHNDPFVSAENTEIPPSVAASGAARNGIPRPKLTTKTNFSGGIQGGISNGAPIYFRVGFKPAATIGQEQTTATYDGTSEGVLAAKGRHDPSVVPRAVPIVEAMAALVIMDAVLAQQARHTAKSLLPPLKQTINSGKDTVGNGVSENVQESDLAQ
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Molecular Weight
46.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Aro-2 recombinant protein is a product of significant interest in biochemical and biotechnological research, stemming from its role in yeast metabolism, particularly in the biosynthesis of aromatic amino acids. Genetic studies have highlighted the Aro-2 enzyme's involvement in the shikimic acid pathway, which is crucial for the production of essential amino acids like phenylalanine, tyrosine, and tryptophan. These amino acids play vital roles in various biological processes, including protein synthesis and neurotransmitter production. The recombinant production of Aro-2, often achieved through genetic engineering techniques in model organisms such as Saccharomyces cerevisiae, allows researchers to study its biochemical properties and reaction mechanisms in detail. This approach not only facilitates the exploration of metabolic engineering for enhanced amino acid production but also has potential applications in pharmaceuticals and agriculture. Understanding Aro-2's structure-function relationship can lead to innovations in developing strains with optimized amino acid profiles. Additionally, studying this protein may provide insights into the regulation of secondary metabolite production in fungi and plants, contributing to our broader understanding of metabolic networks. As a result, the Aro-2 recombinant protein is a focal point for research aimed at bioengineering and optimizing metabolic pathways for sustainable biotechnology applications.
