Analytical Data
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Gene name
RNF43
- Application
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Alternative Names
RNF43；E3 ubiquitin-Protein ligase RNF43
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q68DV7-1
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Expression Region
1-197aa
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AA Sequence
MSGGHQLQLA ALWPWLLMAT LQAGFGRTGL VLAAAVESER SAEQKAIIRV IPLKMDPTGK LNLTLEGVFA GVAEITPAEG KLMQSHPLYL CNASDDDNLE PGFISIVKLE SPRRAPRPCL SLASKARMAG ERGASAVLFD ITEDRAAAEQ LQQPLGLTWP VVLIWGNDAE KLMEFVYKNQ KAHVRIELKE PPAWPDY
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF43 is an E3 ubiquitin ligase that plays a crucial role in regulating the Wnt signaling pathway, which is essential for various biological processes, including cell proliferation, differentiation, and stem cell maintenance. Aberrant activation of Wnt signaling has been implicated in various cancers, particularly colorectal cancer, making RNF43 a significant focus of research in cancer biology. Studies have demonstrated that mutations and misregulation of RNF43 can lead to the stabilization of Wnt pathway components, promoting tumorigenesis. Furthermore, RNF43 has been identified as a potential tumor suppressor, as its loss of function can result in enhanced Wnt signaling and increased cancer cell proliferation. Researchers have sought to develop recombinant RNF43 protein for various applications, including functional assays to elucidate its role in Wnt signaling modulation and as a potential therapeutic target. The understanding of RNF43's function and the mechanisms governing its activity could lead to novel strategies for cancer treatment and offer insights into the broader implications of Wnt signaling in developmental and disease contexts. As such, the study of RNF43 and its recombinant forms represents a vital area of research with substantial potential for impacting cancer therapeutics and enhancing our understanding of cellular signaling pathways.
