Analytical Data
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基因名
CHFR
- Application
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别名
E3 ubiquitin-Protein ligase CHFR; EC 6.3.2.; FLJ10796; FLJ33629; RING finger Protein 196; RNF 116; RNF 196; RNF116; RNF196; Ubiquitin ligase Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96EP1
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表达区间
1-664aa
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氨基酸序列
MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY ESLSEKQGMT QESFEANKEN VFHGTKDTSG AGAGRGADPR VPPSSPATQV CFEEPQPSTS TSDLFPTASA SSTEPSPAGR ERSSSCGSGG GGISPKGSGP SVASDEVSSF ASALPDRKTA SFSSLEPQDQ EDLEPVKKKM RGDGDLDLNG QLLVAQPRRN AQTVHEDVRA AAGKPDKMEE TLTCIICQDL LHDCVSLQPC MHTFCAACYS GWMERSSLCP TCRCPVERIC KNHILNNLVE AYLIQHPDKS RSEEDVQSMD ARNKITQDML QPKVRRSFSD EEGSSEDLLE LSDVDSESSD ISQPYVVCRQ CPEYRRQAAQ PPHCPAPEGE PGAPQALGDA PSTSVSLTTA VQDYVCPLQG SHALCTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCY GCLAPFCELN LGDKCLDGVL NNNSYESDIL KNYLATRGLT WKNMLTESLV ALQRGVFLLS DYRVTGDTVL CYCCGLRSFR ELTYQYRQNI PASELPVAVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT RFKN
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分子量
73.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHFR (Checkpoint with Forkhead and RING finger domains) is a ubiquitin ligase that plays a critical role in the cellular response to DNA damage and the maintenance of genomic stability. Its involvement in the G2/M checkpoint suggests it functions as a tumor suppressor, particularly in cancer biology. Research has shown that CHFR is often downregulated or mutated in various cancers, which correlates with increased cell proliferation and resistance to chemotherapeutic agents. Understanding the structure and function of CHFR, particularly through the production of recombinant CHFR proteins, is essential for unraveling its complex mechanisms in cell cycle regulation and DNA damage response. Previous studies indicate that CHFR interacts with multiple substrates to modulate their stability and function, influencing cell division and the DNA damage repair pathway. The generation of recombinant CHFR proteins enables detailed biochemical and structural studies, facilitating the exploration of its enzymatic activity and interaction partners. This research not only sheds light on the fundamental biology of CHFR but also has implications for therapeutic interventions in malignancies characterized by CHFR dysfunction. By elucidating the molecular basis of CHFR's actions, scientists aim to develop targeted strategies to enhance cancer treatment efficacy and improve patient outcomes in cases where CHFR is implicated.
