Analytical Data
-
Gene name
hlgA
- Application
-
Alternative Names
hlgA；hlg2；Gamma-hemolysin component A
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UI32
-
Expression Region
15-602aa
-
AA Sequence
GSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQDHDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVAAYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
-
Molecular Weight
80.7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the HlgA recombinant protein is rooted in the broader context of microbial pathogenesis and immune response. HlgA, a surface protein found in certain pathogenic strains of *Staphylococcus aureus*, plays a crucial role in the bacteria's ability to evade host immune defenses. This protein is a member of the lactic acid bacteria (LAB) group and is implicated in the formation of biofilms, which are critical for bacterial survival in hostile environments. Given the rise of antibiotic-resistant strains, understanding the molecular mechanisms of how HlgA contributes to virulence and immune evasion is of significant importance. By exploring the structural and functional characteristics of HlgA through recombinant protein techniques, researchers aim to uncover its role in disease processes and its potential as a target for novel therapeutic interventions. Additionally, HlgA's interactions with host cells highlight its significance in the development of vaccines and immune therapies, presenting an exciting avenue for research in the fight against bacterial infections. The recombinant production of HlgA allows for detailed biochemical analysis, facilitating the design of inhibitors and providing insights into how this protein might modulate immune responses. Overall, the investigation of HlgA not only enhances our understanding of *S. aureus* pathogenesis but also contributes to the development of innovative strategies to combat bacterial diseases.
