Analytical Data
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Gene name
RHOBTB3
- Application
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Alternative Names
RHOBTB3; KIAA0878; Rho-related BTB domain-containing protein 3; EC 3.6.1.-
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94955
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Expression Region
1-611 aa
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AA Sequence
MSIHIVALGN EGDTFHQDNR PSGLIRTYLG RSPLVSGDES SLLLNAASTV ARPVFTEYQA SAFGNVKLVV HDCPVWDIFD SDWYTSRNLI GGADIIVIKY NVNDKFSFHE VKDNYIPVIK RALNSVPVII AAVGTRQNEE LPCTCPLCTS DRGSCVSTTE GIQLAKELGA TYLELHSLDD FYIGKYFGGV LEYFMIQALN QKTSEKMKKR KMSNSFHGIR PPQLEQPEKM PVLKAEASHY NSDLNNLLFC CQCVDVVFYN PNLKKVVEAH KIVLCAVSHV FMLLFNVKSP TDIQDSSIIR TTQDLFAINR DTAFPGASHE SSGNPPLRVI VKDALFCSCL SDILRFIYSG AFQWEELEED IRKKLKDSGD VSNVIEKVKC ILKTPGKINC LRNCKTYQAR KPLWFYNTSL KFFLNKPMLA DVVFEIQGTT VPAHRAILVA RCEVMAAMFN GNYMEAKSVL IPVYGVSKET FLSFLEYLYT DSCCPAGIFQ AMCLLICAEM YQVSRLQHIC ELFIITQLQS MPSRELASMN LDIVDLLKKA KFHHSDCLST WLLHFIATNY LIFSQKPEFQ DLSVEERSFV EKHRWPSNMY LKQLAEYRKY IHSRKCRCLV M
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Molecular Weight
69.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RHOBTB3, a member of the Rho GTPase family, has garnered significant attention in recent years due to its crucial roles in cellular processes such as cytoskeletal dynamics, cell migration, and proliferation. Emerging research suggests that RHOBTB3 may also be involved in various pathological conditions, including cancer and neurodegenerative diseases, which raises important questions about its potential as a therapeutic target. Despite its importance, the functional mechanisms and regulatory pathways associated with RHOBTB3 remain inadequately characterized. The production of recombinant RHOBTB3 protein allows for detailed biochemical and structural studies, which are essential for understanding its interactions and functions at the molecular level. Through these studies, researchers aim to elucidate the biological significance of RHOBTB3, explore its potential as a biomarker for disease, and assess its suitability for drug development. Given the complexity of Rho GTPase signaling networks, the recombinant expression and purification of RHOBTB3 is a critical step toward unraveling its role in both healthy and diseased states, ultimately contributing to the advancement of targeted therapies.
