Analytical Data
-
Gene name
DNAJC30
- Application
-
Alternative Names
DNAJC30；WBSCR18；DnaJ homolog subfamily C member 30. mitochondrial
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q96LL9
-
Expression Region
1-226aa
-
AA Sequence
MAAMRWRWWQ RLLPWRLLQA RGFPQNSAPS LGLGARTYSQ GDCSYSRTAL YDLLGVPSTA TQAQIKAAYY RQCFLYHPDR NSGSAEAAER FTRISQAYVV LGSATLRRKY DRGLLSDEDL RGPGVRPSRT PAPDPGSPRT PPPTSRTHDG SRASPGANRT MFNFDAFYQA HYGEQLERER RLRARREALR KRQEYRSMKG LRWEDTRDTA AIFLIFSIFI IIGFYI
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNAJC30, a member of the DnaJ (Hsp40) family of heat shock proteins, plays a pivotal role in cellular protein homeostasis and stress response. This protein is primarily involved in the chaperoning of newly synthesized and misfolded proteins, facilitating their proper folding and preventing aggregation, which can be detrimental to cellular function. The importance of DNAJC30 has been highlighted in various studies linking its expression to neurodegenerative diseases, where protein misfolding is a common pathological feature. Understanding the structure and function of DNAJC30, especially its reactivity under stress conditions, is crucial for unraveling the molecular mechanisms underlying these diseases. Additionally, research has shown that DNAJC30 may interact with specific client proteins, thereby influencing cellular signaling pathways and protein degradation processes. Recent advances in recombinant protein technology have enabled the production and purification of DNAJC30, providing researchers with valuable tools to investigate its functional mechanisms. Studying the recombined DNAJC30 protein offers insights into its chaperone activity and potential therapeutic applications, particularly in strategies aimed at mitigating the effects of protein misfolding in neurodegeneration and other related disorders.
