Analytical Data
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Gene name
Tnni3
- Application
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Alternative Names
Tnni3；TNNC1；Troponin I. cardiac muscle
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48787
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Expression Region
2-211aa
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AA Sequence
ADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG
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Molecular Weight
24.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Tnni3, or troponin I type 3, is a crucial regulatory protein involved in the contraction of cardiac and skeletal muscles. It is part of the troponin complex, which also includes troponin C and troponin T. The primary function of Tnni3 is to bind calcium ions and convey the signal necessary for muscle contraction in response to excitation. Abnormalities in Tnni3 expression or mutations in the gene encoding this protein can lead to various cardiovascular diseases, including hypertrophic cardiomyopathy and dilated cardiomyopathy, making it a significant focus of research. Additionally, Tnni3 serves as a marker for myocardial injury, and its presence in the bloodstream is used to diagnose heart attacks. Consequently, recombinant Tnni3 protein is being studied both for its role in understanding muscle physiology and pathology and for its potential use in therapeutic applications. Research on Tnni3 reconstitution and its interaction with other components of the contractile apparatus aims to elucidate the molecular mechanisms underlying muscle contraction and regulation, offering insights into potential interventions for heart diseases. The generation of recombinant Tnni3 also facilitates the study of the protein's biochemical properties and structural characteristics, allowing researchers to design drugs or therapies that specifically target its pathways, ultimately improving clinical outcomes for patients with cardiac dysfunction.
