Analytical Data
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Gene name
ITK
- Application
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Alternative Names
ITK；EMT；LYK；Tyrosine-Protein kinase ITK/TSK
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08881
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Expression Region
1-620aa
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AA Sequence
MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRT LKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQ RWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTK NASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCL LDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAE KLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETND NPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVT AGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAM SEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLR TQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKV SDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLM WEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKER PEDRPAFSRLLRQLAEIAESGL
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Molecular Weight
108 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ITK (Interleukin-2-inducible T-cell kinase) is a critical protein in the regulation of T-cell signaling and function, particularly in the immune response. Its importance lies in its role as a non-receptor tyrosine kinase that mediates various signaling pathways essential for T-cell activation, differentiation, and survival. Research on ITK has gained momentum due to its implications in various diseases, including autoimmune disorders, chronic inflammation, and certain cancers. Understanding the structure and function of ITK is vital for elucidating its role in T-cell dynamics and for the development of targeted therapies. Recombinant versions of ITK have been engineered to study its interactions with other proteins and to identify potential pharmacological targets. By exploring the biochemical properties and regulatory mechanisms of ITK, researchers aim to harness its therapeutic potential, providing new avenues for treating conditions linked to immune dysregulation. This research not only contributes to the basic understanding of T-cell biology but also holds promise for innovative treatment strategies in immunotherapy.
