Analytical Data
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Gene name
RASEF
- Application
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Alternative Names
RASEF; RAB45; Ras and EF-hand domain-containing protein; Ras-related protein Rab-45
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IZ41
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Expression Region
1-740 aa
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AA Sequence
MEADGDGEEL ARLRSVFAAC DANRSGRLER EEFRALCTEL RVRPADAEAV FQRLDADRDG AITFQEFARG FLGSLRGGRR RDWGPLDPAP AVSEAGPETH DSEEDEGDED AAAALATSCG PASPGRAWQD FQARLGDEAK FIPREEQVST LYQNINLVEP RLIQPYEHVI KNFIREIRLQ STEMENLAIA VKRAQDKAAM QLSELEEEMD QRIQAAEHKT RKDEKRKAEE ALSDLRRQYE TEVGDLQVTI KKLRKLEEQS KRVSQKEDVA ALKKQIYDLS MENQKVKKDL LEAQTNIAFL QSELDALKSD YADQSLNTER DLEIIRAYTE DRNSLERQIE ILQTANRKLH DSNDGLRSAL ENSYSKFNRS LHINNISPGN TISRSSPKFI GHSPQPLGYD RSSRSSYVDE DCDSLALCDP LQRTNCEVDS LPESCFDSGL STLRDPNEYD SEVEYKHQRG FQRSHGVQES FGGDASDTDV PDIRDEETFG LEDVASVLDW KPQGSVSEGS IVSSSRKPIS ALSPQTDLVD DNAKSFSSQK AYKIVLAGDA AVGKSSFLMR LCKNEFRENI SATLGVDFQM KTLIVDGERT VLQLWDTAGQ ERFRSIAKSY FRKADGVLLL YDVTCEKSFL NIREWVDMIE DAAHETVPIM LVGNKADIRD TAATEGQKCV PGHFGEKLAM TYGALFCETS AKDGSNIVEA VLHLAREVKK RTDKDDSRSI TNLTGTNSKK SPQMKNCCNG
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Molecular Weight
82.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
RASEF, or Ras superfamily of small GTPases, is a critical protein family that plays significant roles in various cellular processes, including signal transduction, cell growth, and differentiation. The research surrounding RASEF has gained momentum due to its implications in cancer biology and other diseases. RASEF is recognized for its ability to regulate key pathways by acting as a molecular switch, toggling between an active GTP-bound state and an inactive GDP-bound state. Dysregulation of RASEF and its associated pathways has been linked to tumorigenesis and metastasis, making it a potential target for therapeutic intervention. Recent studies have focused not only on the functional characterization of RASEF but also on developing recombinant proteins for further exploration of its regulatory mechanisms. By generating and purifying RASEF as a recombinant protein, researchers can better understand its structure-function relationships, investigate its interactions with other proteins, and ultimately contribute to the development of novel cancer treatments. The advancement of technologies in protein expression and purification has played a vital role in enabling scientists to conduct detailed investigations into RASEF's molecular dynamics. This ongoing research underscores the importance of RASEF in cellular communication and highlights its potential as a candidate for targeted therapies in combating cancer and other related diseases.
