Analytical Data
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Gene name
B4GALT3
- Application
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Alternative Names
B4GALT3；Beta-1.4-galactosyltransferase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60512
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Expression Region
32-393aa
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AA Sequence
RSLSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERS PLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPH RAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVRE ALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYP QYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPT SVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLAREL GPLYTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPL STANHTALRGSHVDHHHHHH
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Molecular Weight
42 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
B4GALT3, a member of the beta-1,4-galactosyltransferase family, plays a crucial role in glycosylation processes, specifically in the synthesis of glycosphingolipids and glycoproteins. It catalyzes the transfer of galactose to specific sugar residues, influencing important cellular functions such as adhesion, signaling, and immune responses. Research on B4GALT3 is significant due to its potential implications in various diseases, including cancer, where altered glycosylation patterns can affect tumor progression and metastasis. Furthermore, mutations in the B4GALT3 gene have been linked to congenital disorders, highlighting its importance in maintaining normal cellular functions. The functional characterization of B4GALT3 through recombinant protein studies allows for a better understanding of its enzymatic activity, substrate specificity, and regulatory mechanisms. This helps in elucidating its physiological roles and exploring potential therapeutic strategies for diseases associated with abnormal glycosylation. As such, investigating B4GALT3 not only enhances our knowledge of glycosylation biology but also paves the way for novel approaches in medical research and drug development.
