Analytical Data
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Gene name
Nr5a2
- Application
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Alternative Names
Nr5a2；B1F；CPF；FTF；Nuclear receptor subfamily 5 group A member 2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00482
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Expression Region
1-541aa
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AA Sequence
MSSNSDTGDL QESLKHGLTP IGAGLPDRHG SPIPARGRLV MLPKVETEAL GLARSHGEQG QMPENMQVSQ FKMVNYSYDE DLEELCPVCG DKVSGYHYGL LTCESCKGFF KRTVQNNKRY TCIENQNCQI DKTQRKRCPY CRFQKCLSVG MKLEAVRADR MRGGRNKFGP MYKRDRALKQ QKKALIRANG LKLEAMSQVI QAMPSDLTIS SAIQNIHSAS KGLPLNHAAL PPTDYDRSPF VTSPISMTMP PHGSLQGYQT YGHFPSRAIK SEYPDPYTSS PESIMGYSYM DSYQTSSPAS IPHLILELLK CEPDEPQVQA KIMAYLQQEQ ANRSKHEKLS TFGLMCKMAD QTLFSIVEWA RSSIFFRELK VDDQMKLLQN CWSELLILDH IYRQVVHGKE GSIFLVTGQQ VDYSIIASQA GATLNNLMSH AQELVAKLRS LQFDQREFVC LKFLVLFSLD VKNLENFQLV EGVQEQVNAA LLDYTMCNYP QQTEKFGQLL LRLPEIRAIS MQAEEYLYYK HLNGDVPYNN LLIEMLHAKR A
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The Nr5a2 (Nuclear Receptor Subfamily 5 Group A Member 2), also known as liver receptor homolog-1 (LRH-1), is a nuclear receptor that plays a crucial role in various biological processes including lipid metabolism, steroidogenesis, and embryonic development. Its expression is notably prominent in the liver, pancreas, and gonads, indicating its significance in metabolic regulation and endocrine functions. Dysregulation of Nr5a2 has been implicated in several diseases, including obesity, diabetes, and certain cancers, making it an attractive target for therapeutic interventions. Research has increasingly focused on the structural and functional aspects of Nr5a2, particularly its potential as a biomarker and therapeutic target. Recombinant proteins of Nr5a2 are being engineered to study its binding affinity to specific ligands and its interaction with coregulators, which are crucial for understanding its regulatory mechanisms. Such studies can provide insights into how Nr5a2 modulates gene expression in response to various physiological stimuli, enhancing our understanding of metabolic disorders and developing innovative strategies for treatment. Furthermore, the advancement of techniques such as X-ray crystallography and NMR spectroscopy has facilitated a deeper exploration of the three-dimensional structure of Nr5a2, allowing researchers to identify key amino acid residues that contribute to its function. This structural information is essential for designing small molecules or peptides that can selectively modulate Nr5a2 activity, paving the way for novel pharmacological approaches that could lead to significant improvements in the management of metabolic diseases linked to Nr5a2 dysregulation.
