Analytical Data
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基因名
PADI4
- Application
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别名
PADI4；PAD4；PADI5；PDI5；Protein-arginine deiminase type-4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UM07
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表达区间
1-663aa
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氨基酸序列
MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVV DIAHGPPAKK KSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYG PKTPPVKALLYLTGVEISLCAD ITRTGKVKPTRAVKDQRTWTWGPCGQ GAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDM SLMTLSTKTPKDFF TNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMV PG GKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFR VAPWIMTP NTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEE ENMDDQWMQDEMEIGYIQAP HKTLPVVFDSPRNRGLKEFPIKRVMGPD FGYVTRGPQTGGISGLDSFGNLEVSPPVTVRG KEYPLGRILFGDSCYP SNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVP APDR KGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKT LREHNS FVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAF FPNMVNMLVLGKHLGIPK PFGPVINGRCCLEEKVCSLLEPLGLQCTFI NDFFTYHIRHGEVHCGTNVRRKPFSFKWWN MVP
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分子量
75 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PADI4 (Peptidylarginine deiminase type 4) is an enzyme that converts arginine residues in proteins into citrulline, a process known as citrullination. This post-translational modification has significant implications in various biological processes, including the regulation of gene expression, protein stability, and immune responses. Dysregulation of PADI4 has been linked to several autoimmune diseases, collectively termed as citrullination-related diseases, such as rheumatoid arthritis and multiple sclerosis. Research into PADI4's structure and function is critical, as understanding its mechanistic role in citrullination can provide insights into disease pathogenesis and potential therapeutic targets. Recent studies have employed techniques such as X-ray crystallography and cryo-electron microscopy to elucidate the enzyme's structure, revealing insights into its catalytic mechanism and substrate specificity. Moreover, PADI4's interaction with specific proteins and signaling pathways is being investigated to understand its broader impact on cellular functions and disease states. Given its pivotal role in modulating inflammatory processes, PADI4 is emerging as a promising target for drug development, aiming to inhibit its activity in pathological conditions where excessive citrullination contributes to disease progression. Hence, PADI4 research not only deepens our understanding of protein modification but also holds potential for advancing therapeutic interventions in autoimmune disorders.
