Analytical Data
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基因名
MMUT
- Application
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别名
MMUT；MUT；Methylmalonyl-CoA mutase. mitochondrial
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P22033
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表达区间
33-750aa
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氨基酸序列
LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV
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分子量
84.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MMUT (methylmalonyl-CoA mutase) is an essential enzyme involved in the catabolism of branched-chain amino acids and the metabolism of certain fatty acids. Deficiency in MMUT activity can lead to methylmalonic acidemia (MMA), a metabolic disorder characterized by the accumulation of toxic levels of methylmalonic acid in the body, resulting in significant health issues, including neurological impairment and metabolic crises. Research into recombinant MMUT proteins aims to understand the enzyme's structure-function relationship, gain insights into the molecular basis of MMUT-related disorders, and explore potential therapeutic interventions. The expression and purification of recombinant MMUT can provide ample quantities of the enzyme for biochemical studies, allowing researchers to investigate the effects of various mutations that lead to MMA. Furthermore, recombinant MMUT represents a promising avenue for developing enzyme replacement therapies, offering hope for affected individuals by restoring normal metabolic function. By leveraging advancements in recombinant DNA technology, researchers are focusing on characterizing the enzyme's kinetic properties, studying its interactions with substrates and cofactors, and exploring potential small-molecule chaperones that could enhance MMUT stability and function. Overall, the study of MMUT and its recombinant forms is a critical field of inquiry that holds promise not only for understanding the biochemistry underlying MMA but also for developing innovative strategies to treat this challenging metabolic disorder.
