Analytical Data
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Gene name
MMUT
- Application
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Alternative Names
MMUT；MUT；Methylmalonyl-CoA mutase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P22033
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Expression Region
33-750aa
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AA Sequence
LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV
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Molecular Weight
84.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MMUT (methylmalonyl-CoA mutase) is an essential enzyme involved in the catabolism of branched-chain amino acids and the metabolism of certain fatty acids. Deficiency in MMUT activity can lead to methylmalonic acidemia (MMA), a metabolic disorder characterized by the accumulation of toxic levels of methylmalonic acid in the body, resulting in significant health issues, including neurological impairment and metabolic crises. Research into recombinant MMUT proteins aims to understand the enzyme's structure-function relationship, gain insights into the molecular basis of MMUT-related disorders, and explore potential therapeutic interventions. The expression and purification of recombinant MMUT can provide ample quantities of the enzyme for biochemical studies, allowing researchers to investigate the effects of various mutations that lead to MMA. Furthermore, recombinant MMUT represents a promising avenue for developing enzyme replacement therapies, offering hope for affected individuals by restoring normal metabolic function. By leveraging advancements in recombinant DNA technology, researchers are focusing on characterizing the enzyme's kinetic properties, studying its interactions with substrates and cofactors, and exploring potential small-molecule chaperones that could enhance MMUT stability and function. Overall, the study of MMUT and its recombinant forms is a critical field of inquiry that holds promise not only for understanding the biochemistry underlying MMA but also for developing innovative strategies to treat this challenging metabolic disorder.
