Analytical Data
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Gene name
SEMA4C
- Application
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Alternative Names
SEMA4C；KIAA1739；SEMAI；Semaphorin-4C
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Species
Mouse
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q64151
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Expression Region
21-664aa
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AA Sequence
AEMWWNLVPRKTVSSGELVTVVRRFSQTGIQDFLTLTLTEHSGLLYVGAREALFAFSVEALELQGAISWEAPAEKKIECTQKGKSNQTECFNFIRFLQPYNSSHLYVCGTYAFQPKCTYINMLTFTLDRAEFEDGKGKCPYDPAKGHTGLLVDGELYSATLNNFLGTEPVILRYMGTHHSIKTEYLAFWLNEPHFVGSAFVPESVGSFTGDDDKIYFFFSERAVEYDCYSEQVVARVARVCKGDMGGARTLQKKWTTFLKARLVCSAPDWKVYFNQLKAVHTLRGASWHNTTFFGVFQARWGDMDLSAVCEYQLEQIQQVFEGPYKEYSEQAQKWARYTDPVPSPRPGSCINNWHRDNGYTSSLELPDNTLNFIKKHPLMEDQVKPRLGRPLLVKKNTNFTHVVADRVPGLDGATYTVLFIGTGDGWLLKAVSLGPWIHMVEELQVFDQEPVESLVLSQSKKVLFAGSRSQLVQLSLADCTKYRFCVDCVLARDPYCAWNVNTSRCVATTS
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Molecular Weight
100.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SEMA4C, a member of the semaphorin family, plays a significant role in mediating cellular interactions during developmental processes and in the nervous system. This transmembrane protein is known to influence axon guidance, neuronal differentiation, and immune responses through its interactions with various receptors, notably plexins and neuropilins. Abnormal expression of SEMA4C has been implicated in several pathological conditions, including cancer and neurological disorders, making it a potential target for therapeutic interventions. Recent studies have focused on the recombinant expression and purification of SEMA4C to better understand its structure-function relationships. By utilizing advanced techniques such as protein engineering and crystallography, researchers aim to elucidate the molecular mechanisms by which SEMA4C exerts its biological effects. Additionally, generating recombinant SEMA4C proteins provides a platform for exploring their role in cell signaling pathways and for screening potential inhibitors or modulators. The ongoing research into SEMA4C holds promise for developing novel strategies in treating diseases where semaphorin signaling is disrupted, thus paving the way for significant advancements in both basic biology and clinical applications.
