Analytical Data
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Gene name
VCP
- Application
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Alternative Names
VCP；Small VCP/p97-interacting Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P55072
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Expression Region
1-806aa
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AA Sequence
MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQ LFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVI SIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGD IFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNE VGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTG KTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAI IFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRP NSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVA NETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMD DFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHP DKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTM WFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRV INQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDE KSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIR ESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDN DIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDN DDDLYG
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Molecular Weight
116 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
VCP (Valosin-containing protein), also known as p97, is a member of the AAA ATPase family and plays a critical role in various cellular processes, including protein degradation, membrane fusion, and cell cycle regulation. Dysfunction of VCP is associated with multiple human diseases, including frontotemporal dementia, inclusion body myopathy, and amyotrophic lateral sclerosis (ALS). Given its significant implications in these pathologies, the study of VCP has garnered considerable attention in the fields of molecular biology and biochemistry. Researchers have focused on understanding its structure-function relationship and the mechanisms by which VCP interacts with various substrates and cofactors. The production of recombinant VCP proteins through techniques such as bacterial or mammalian cell expression systems has become essential for these studies, enabling scientists to investigate its biochemical properties and regulatory mechanisms in a controlled environment. This research is pivotal not only for elucidating the normal physiological roles of VCP but also for developing potential therapeutic strategies targeting its dysfunction in disease contexts. Furthermore, recombinant VCP proteins can serve as valuable tools in drug discovery and the screening of small molecules that may modulate its activity, presenting new avenues for treatment options in VCP-related diseases. Overall, the investigation of VCP and its recombinant forms represents a promising frontier in understanding key cellular processes and addressing the challenges posed by neurodegenerative conditions linked to its malfunction.
