Analytical Data
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Gene name
KDM5A
- Application
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Alternative Names
KDM5A；JARID1A；RBBP2；RBP2；Lysine-specific demethylase 5A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29375
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Expression Region
1-1090aa
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AA Sequence
MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICK IRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWEL QGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLP GKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTD TQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVG LAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCG RGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPRE AFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSS IEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPV LEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEP KTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGV PVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVN HYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLR ESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCL YHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEAL SANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQL LLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLL DDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQ ARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLT VSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQK AREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAA RAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRR
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Molecular Weight
125 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KDM5A, also known as lysine-specific demethylase 5A, is a member of the Jumonji C domain-containing family of proteins and plays a crucial role in the regulation of gene expression through its histone demethylase activity. It specifically demethylates tri- and di-methylated lysine 4 on histone H3 (H3K4me3 and H3K4me2), which are marks associated with active transcription. Dysfunction or aberrant expression of KDM5A has been linked to several types of cancer, including breast, prostate, and lung cancers, highlighting its potential as a therapeutic target. The study of KDM5A recombinant proteins allows researchers to dissect its structural and functional properties, providing insights into the mechanisms by which it regulates chromatin dynamics and gene expression. Furthermore, understanding the interactions between KDM5A and other cellular proteins can unveil its role in various signaling pathways and its implications in disease states. Recent advances in protein engineering have enabled the production of large quantities of KDM5A recombinant proteins, which facilitate biochemical assays, structural studies, and the development of small-molecule inhibitors. This research ultimately aims to advance our understanding of epigenetic regulation and explore novel strategies for targeted therapy in cancers associated with KDM5A dysregulation.
