Analytical Data
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Gene name
EIF4G1
- Application
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Alternative Names
EIF4G1;EIF4F;EIF4G;EIF4GI;Eukaryotic translation initiation factor 4 gamma 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q04637
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Expression Region
1250-1599aa
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AA Sequence
IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQ LLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQ EGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREA GLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEK LLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRV DVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDAL YDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN
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Molecular Weight
47 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma 1) is a crucial component of the eukaryotic translation initiation machinery, playing a significant role in protein synthesis regulation. It serves as a scaffold for the assembly of various initiation factors and is pivotal for the recruitment of ribosomes to the mRNA. Research has identified EIF4G1 as a central player in cellular processes beyond translation, such as cell growth and differentiation. Dysregulation of EIF4G1 has been implicated in various diseases, including cancer, where it may contribute to the uncontrolled proliferation of cells through enhanced protein synthesis. As a result, EIF4G1 has garnered attention as a potential therapeutic target. Recent studies have focused on characterizing its structure and function, exploring its interactions with other proteins, and investigating its role in different cellular contexts. Understanding the intricacies of EIF4G1's activity could lead to novel strategies for treating diseases associated with translation dysregulation, making it a significant subject of ongoing research in molecular biology and translational medicine.











