Analytical Data
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Gene name
EIF2AK2
- Application
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Alternative Names
EIF2AK2；PKR；PRKR；Interferon-induced. double-stranded RNA-activated Protein kinase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19525
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Expression Region
2-551aa
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AA Sequence
AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC
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Molecular Weight
64.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EIF2AK2, also known as PKR (Protein Kinase R), is a serine/threonine kinase that plays a crucial role in the cellular response to viral infections and other stressors by regulating protein synthesis and inducing apoptosis. It is activated in response to double-stranded RNA, a common viral replication intermediate, leading to the phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α), which inhibits protein translation. This mechanism is vital for limiting viral replication and enhancing the host's immune response. Given the importance of EIF2AK2 in the innate immune response, researchers are focusing on its potential as a therapeutic target for viral infections and cancer. The recombinant expression of EIF2AK2 allows for in-depth studies into its structure, function, and interaction with other cellular pathways. This research aims to elucidate the role of EIF2AK2 in modulating gene expression and its involvement in various pathological conditions, including autoimmune disorders and malignancies. By investigating the properties and mechanisms of this protein, scientists hope to identify new strategies for manipulating the immune response and developing novel antiviral or anticancer therapies. Understanding EIF2AK2’s functions at a molecular level could pave the way for innovative treatments that enhance immune responses or disrupt the ability of pathogens to evade host defenses. Overall, the study of EIF2AK2 not only enriches our knowledge of cellular stress responses but also holds promise for the development of targeted therapies in infectious diseases and cancer.
