Analytical Data
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Gene name
ST8SIA1
- Application
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Alternative Names
ST8SIA1；SIAT8；SIAT8A；Alpha-N-acetylneuraminide alpha-2.8-sialyltransferase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q92185-1
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Expression Region
49-356aa
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AA Sequence
YRLPNEKEIV QGVLQQGTAW RRNQTAARAF RKQMEDCCDP AHLFAMTKMN SPMGKSMWYD GEFLYSFTID NSTYSLFPQA TPFQLPLKKC AVVGNGGILK KSGCGRQIDE ANFVMRCNLP PLSSEYTKDV GSKSQLVTAN PSIIRQRFQN LLWSRKTFVD NMKIYNHSYI YMPAFSMKTG TEPSLRVYYT LSDVGANQTV LFANPNFLRS IGKFWKSRGI HAKRLSTGLF LVSAALGLCE EVAIYGFWPF SVNMHEQPIS HHYYDNVLPF SGFHAMPEEF LQLWYLHKIG ALRMQLDPCE DTSLQPTS
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Molecular Weight
37 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ST8SIA1 gene encodes a sialyltransferase enzyme primarily involved in the biosynthesis of sialylated glycoproteins and glycolipids, which are critical for various biological processes, including cell adhesion, signaling, and immune response. Abnormal expression of ST8SIA1 has been linked to several pathological conditions, including cancer and neurodegenerative diseases. Researchers have increasingly focused on the functional role of the ST8SIA1 protein, particularly in relation to its impact on cellular interactions and molecular pathways. Understanding the molecular mechanisms by which ST8SIA1 regulates sialylation could provide insights into its involvement in disease progression and potential therapeutic targets. The generation of recombinant ST8SIA1 protein has facilitated detailed studies of its enzymatic properties and substrate specificity, thereby advancing knowledge of its biological functions. These investigations not only enhance our understanding of sialylation processes but also pave the way for the development of novel strategies for disease diagnosis and treatment, highlighting the importance of ST8SIA1 in both basic and applied biomedical research.
