Analytical Data
-
Gene name
ADH1B
- Application
-
Alternative Names
ADH1B；ADH2；All-trans-retinol dehydrogenase [NAD(+)] ADH1B
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P00325
-
Expression Region
1-375aa
-
AA Sequence
MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTD DHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGK CRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQY TVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFG LGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKP IQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQ NLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHV LPFEKINEGFDLLHSGKSIRTVLTF
-
Molecular Weight
67 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ADH1B (Alcohol Dehydrogenase 1B) is a crucial enzyme involved in the metabolism of alcohol in the human liver. Variants of this gene have been linked to differences in alcohol sensitivity and susceptibility to alcohol-related disorders. The ADH1B*2 allele, specifically, is associated with higher enzymatic activity, leading to increased acetaldehyde production from ethanol metabolism. This heightened activity can result in unpleasant physiological effects, thereby reducing alcohol consumption among individuals carrying this variant. Understanding the functional properties and regulation of ADH1B is essential for elucidating the genetic factors influencing alcohol metabolism and its related health impacts. Moreover, recombinant ADH1B can serve as a valuable tool in pharmaceutical research for drug development and understanding metabolic pathways involving alcohol. Recent studies have focused on producing and characterizing recombinant ADH1B proteins to explore their kinetic properties, substrate specificity, and interaction with inhibitors. This research not only aids in understanding the enzyme's role in alcohol metabolism but also has implications for developing therapeutic strategies for alcohol use disorders and other related conditions. The increasing interest in precision medicine and personalized treatments underscores the importance of such studies in tailoring interventions based on an individual's genetic makeup.
