Analytical Data
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Gene name
DCP1B
- Application
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Alternative Names
DCP1B；mRNA-decapping enzyme 1B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IZD4
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Expression Region
2-617aa
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AA Sequence
AAVAAGGLVGKGRDISLAALQRHDPYINRIVDVASQVALYTFGHRANEWEKTDVEGTLFVYTRSASPKHGFTIMNRLSMENRTEPITKDLDFQLQDPFLLYRNARLSIYGIWFYDKEECQRIAELMKNLTQYEQLKAHQGTGAGISPVILNSGEGKEVDILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKPSENQQQRIPQPNQTLDPEPQHLSLTALFGKQDKATCQETVEPPQTLHQQQQQQQQQQEKLPIRQGVVRSLSYEEPRRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGSTHSAGEFFTGPVQPGSPHNIGTSRGVQNASRTQNLFEKLQSTPGAANKCDPSTPAPASSAALNRSRAPTSVTPVAPGKGLAQPPQAYFNGSLPPQTVGHQAHGREQSTLPRQTLPISGSQTGSSGVISPQELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKPLESWINKTPNTEQQTPLFQVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQEPPAAATSLLLPIQSPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQAAMKKTM
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Molecular Weight
69.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCP1B (Decapping mRNA 1B) is a protein that plays a crucial role in the process of mRNA decay, an essential mechanism for regulating gene expression in eukaryotic cells. It is part of the decapping complex that removes the cap structure at the 5' end of mRNA, facilitating subsequent degradation by exonucleases. Understanding the function and regulation of DCP1B is vital for elucidating the intricacies of RNA metabolism and cellular homeostasis. Dysregulation of mRNA decay pathways has been implicated in various diseases, including cancers and neurodegenerative disorders, making DCP1B a potential target for therapeutic interventions. Recent studies have highlighted its interactions with other RNA-binding proteins and factors involved in mRNA processing and translation, revealing a complex network influencing RNA stability and turnover. Investigating the biochemical properties and structural characteristics of recombinant DCP1B enhances our understanding of its role within the decapping machinery and its contribution to post-transcriptional regulation. Additionally, research into DCP1B may provide insights into the dynamic responses of cells to stress, infection, and developmental signals, further establishing its significance in cellular physiology. Thus, the study of DCP1B not only contributes to our fundamental understanding of molecular biology but also holds promise for therapeutic advancements in treating diseases associated with aberrant mRNA metabolism.
