Analytical Data
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Gene name
CNGA4
- Application
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Alternative Names
CNGA4；Cyclic nucleotide-gated channel alpha-4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IV77
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Expression Region
1-575aa
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AA Sequence
MSQDTKVKTTESSPPAPSKARKLLPVLDPSGDYYYWWLNTMVFPVMYNLI ILVCRACFPDLQHGYLVAWLVLDYTSDLLYLLDMVVRFHTGFLEQGILVV DKGRISSRYVRTWSFFLDLASLMPTDVVYVRLGPHTPTLRLNRFLRAPRL FEAFDRTETRTAYPNAFRIAKLMLYIFVVIHWNSCLYFALSRYLGFGRDA WVYPDPAQPGFERLRRQYLYSFYFSTLILTTVGDTPPPAREEEYLFMVGD FLLAVMGFATIMGSMSSVIYNMNTADAAFYPDHALVKKYMKLQHVNRKLE RRVIDWYQHLQINKKMTNEVAILQHLPERLRAEVAVSVHLSTLSRVQIFQ NCEASLLEELVLKLQPQTYSPGEYVCRKGDIGQEMYIIREGQLAVVADDG ITQYAVLGAGLYFGEISIINIKGNMSGNRRTANIKSLGYSDLFCLSKEDL REVLSEYPQAQTIMEEKGREILLKMNKLDVNAEAAEIALQEATESRLRGL DQQLDDLQTKFARLLAELESSALKIAYRIERLEWQTREWPMPEDLAEADD EGEPEEGTSKDEEGRASQEGPPGPE
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Molecular Weight
66 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The CNGA4 gene encodes a subunit of cyclic nucleotide-gated (CNG) channels, which play a critical role in sensory transduction, particularly in photoreceptor and olfactory neurons. Research on CNGA4 has gained importance due to its involvement in various physiological processes and potential links to certain sensory disorders. The CNG channels, composed of multiple subunits, allow the passage of cations in response to binding of cyclic nucleotides like cGMP, influencing neuronal excitability and signal transmission. Mutations in CNGA4 have been associated with retinitis pigmentosa and other inherited retinal diseases, highlighting the need for in-depth functional studies of this protein. Recombinant CNGA4 protein serves as a valuable tool for exploring its biophysical properties, channel kinetics, and interactions with other signaling molecules. Additionally, characterizing the recombinant protein can shed light on its role in normal sensory functions and its alterations in disease states. Thus, studies focusing on the expression, purification, and functional analysis of CNGA4 reconstituted in heterologous systems contribute significantly to our understanding of its physiological relevance and therapeutic potential in treating sensory impairments.
