Analytical Data
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Gene name
EMC4
- Application
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Alternative Names
EMC4；TMEM85；ER membrane Protein complex subunit 4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5J8M3
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Expression Region
2-183aa
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AA Sequence
TAQGGLVANR GRRFKWAIEL SGPGGGSRGR SDRGSGQGDS LYPVGYLDKQ VPDTSVQETD RILVEKRCWD IALGPLKQIP MNLFIMYMAG NTISIFPTMM VCMMAWRPIQ ALMAISATFK MLESSSQKFL QGLVYLIGNL MGLALAVYKC QSMGLLPTHA SDWLAFIEPP ERMEFSGGGL LL
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Molecular Weight
26 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EMC4 (Endoplasmic Membrane Complex 4) is a member of the EMC protein family, which plays a crucial role in the biogenesis and proper functioning of multi-spanning transmembrane proteins within the endoplasmic reticulum (ER). Research on EMC4 has gained attention due to its involvement in various cellular processes, including protein folding, quality control, and maturation of membrane proteins, which are essential for cell signaling and homeostasis. Aberrations in EMC4 function can lead to a range of diseases, particularly those related to protein misfolding and ER stress, such as neurodegenerative disorders and certain cancers. Understanding the structural and functional properties of EMC4 is vital for elucidating its role in these processes. Recent studies have focused on characterizing the protein's structure, interactions with other ER components, and its mechanism in assisting the integration of new proteins into the ER membrane. This research not only contributes to our fundamental understanding of cellular biology but also holds potential therapeutic implications, as targeting EMC4 pathways may provide novel strategies for treating diseases linked to its dysfunction. Furthermore, advances in cryo-electron microscopy and other structural biology techniques have allowed for unprecedented insights into the composition and dynamics of the EMC complex, paving the way for future discoveries about membrane protein biogenesis and the overall functioning of the endoplasmic reticulum.
