Analytical Data
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基因名
WNT3A
- Application
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别名
WNT3A；Protein Wnt-3a
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P56704-2
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表达区间
1-385aa
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氨基酸序列
MAPLGYFLLLCSLKQALGSYPIWWSLAVGPQYSSLGSQPILCASIPGLVP KQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGP VLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGW KWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMH LKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESR GWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVS SHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHT CKNPGSRAGNSAHQPPHPQPPVRFHPPLRRAGKVP
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分子量
69 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WNT3A is a member of the Wnt family of glycoproteins that play crucial roles in embryonic development and cellular processes such as proliferation, differentiation, and cell fate determination. The Wnt signaling pathway, particularly the canonical pathway activated by WNT ligands like WNT3A, has been extensively studied for its involvement in various biological mechanisms and its implications in diseases, including cancer and degenerative disorders. Research on recombinant WNT3A protein has garnered attention due to its potential therapeutic applications, especially in regenerative medicine and tissue engineering. By investigating the effects of WNT3A on stem cell behavior and tissue regeneration, scientists aim to harness its signaling capabilities to promote tissue repair and enhance stem cell differentiation. Additionally, recombinant WNT3A is used in in vitro studies to better understand the molecular mechanisms underlying Wnt signaling and its interactions with other pathways. The production of high-quality, bioactive recombinant WNT3A enables researchers to explore its functional roles more effectively, leading to advances in both basic biology and clinical applications.
