Analytical Data
-
Gene name
WLS
- Application
-
Alternative Names
WLS；C1orf139；Protein wntless homolog
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q5T9L3
-
Expression Region
101-232aa
-
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK
-
Molecular Weight
18 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
WLS (Wntless) is an essential protein involved in the Wnt signaling pathway, which plays a crucial role in various developmental processes, including cell proliferation, differentiation, and migration. Wnt proteins are lipid-modified secreted ligands that are vital for cellular communication during embryonic development and tissue homeostasis. WLS is responsible for the transport of these Wnt proteins from their site of synthesis in the endoplasmic reticulum to the plasma membrane, facilitating their secretion. Disruptions in Wnt signaling have been implicated in numerous diseases, particularly in cancers and developmental disorders. Consequently, understanding the mechanism of WLS and its interaction with Wnt proteins is of great interest to researchers. The study of WLS includes investigating its structure, function, and the molecular machinery involved in the Wnt export process, as well as its regulatory mechanisms. Recent advancements in recombinant protein expression and purification techniques have enabled researchers to produce WLS in a more streamlined manner, aiding in the detailed biochemical characterization of this protein. Through these studies, scientists hope to uncover new therapeutic targets for diseases associated with Wnt signaling disruptions, thereby highlighting the importance of WLS in both fundamental biology and clinical research.
