Analytical Data
-
Gene name
ISM1
- Application
-
Alternative Names
ISM1；C20orf82；ISM；Isthmin-1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
B1AKI9
-
Expression Region
30-464aa
-
AA Sequence
ADGPDAAAGNASQAQLQNNLNVGSDTTSETSFSLSKEAPREHLDHQAAHQ PFPRPRFRQETGHPSLQRDFPRSFLLDLPNFPDLSKADINGQNPNIQVTI EVVDGPDSEADKDQHPENKPSWSVPSPDWRAWWQRSLSLARANSGDQDYK YDSTSDDSNFLNPPRGWDHTAPGHRTFETKDQPEYDSTDGEGDWSLWSVC SVTCGNGNQKRTRSCGYACTATESRTCDRPNCPGIEDTFRTAATEVSLLA GSEEFNATKLFEVDTDSCERWMSCKSEFLKKYMHKVMNDLPSCPCSYPTE VAYSTADIFDRIKRKDFRWKDASGPKEKLEIYKPTARYCIRSMLSLESTT LAAQHCCYGDNMQLITRGKGAGTPNLISTEFSAELHYKVDVLPWIICKGD WSRYNEARPPNNGQKCTESPSDEDYIKQFQEAREY
-
Molecular Weight
69 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ISM1 is a member of the imprinted gene family, primarily expressed in the mammalian testis and plays a pivotal role in reproductive biology. Its research background is rooted in the understanding of genomic imprinting, a genetic phenomenon where certain genes are expressed in a parent-of-origin-specific manner. Imprinted genes like ISM1 are crucial for regulating growth and development in mammals. Recent studies have highlighted ISM1's potential involvement in male fertility and spermatogenesis, as its expression is linked to sperm quality and function. Furthermore, alterations in the expression of ISM1 have been implicated in various reproductive disorders and developmental abnormalities, making it an essential candidate for studying the molecular mechanisms underlying these conditions. The advent of recombinant protein technology has enabled researchers to produce ISM1 in a laboratory setting, facilitating the investigation of its structural and functional properties. By characterizing ISM1's protein interactions and biological activity, scientists aim to uncover its roles in spermatogenic processes and its implications in reproductive health. This research not only enhances the understanding of the genetic factors contributing to male infertility but also opens avenues for potential therapeutic interventions in managing reproductive challenges related to imprinted gene dysregulation. Overall, the study of recombinant ISM1 protein is poised to provide critical insights into the complex interplay between genetics and reproduction, positioning it as a significant focus in the fields of reproductive biology and molecular genetics.
