Analytical Data
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Gene name
ASCL3
- Application
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Alternative Names
ASCL3; BHLHA42; HASH3; SGN1Achaete-scute homolog 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQ33
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Expression Region
1-180aa
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AA Sequence
MDNRGNSSLP DKLPIFPDSA RLPLTRSFYL EPMVTFHVHP EAPVSSPYSE ELPRLPFPSD SLILGNYSEP CPFSFPMPYP NYRGCEYSYG PAFTRKRNER ERQRVKCVNE GYAQLRHHLP EEYLEKRLSK VETLRAAIKY INYLQSLLYP DKAETKNNPG KVSSMIATTS HHADPMFRIV
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Molecular Weight
20 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ASCL3, or Achaete-Scute Family BHLH Transcription Factor 3, is a member of the basic helix-loop-helix (bHLH) transcription factor family, playing a crucial role in the development of various tissues and cell types. It is primarily involved in neurogenesis, influencing neuronal lineage specification and differentiation. Studies have shown that ASCL3 is essential for the generation of specific neuronal populations during embryonic development, underscoring its significance in central nervous system formation. Additionally, ASCL3 has been implicated in the regulation of gene expression associated with stem cell maintenance and lineage commitment. Given its pivotal functions, researchers have focused on producing recombinant ASCL3 protein to investigate its structure-function relationship, regulatory mechanisms, and potential interactions with other proteins in signaling pathways. Understanding ASCL3's role at the molecular level can provide insights into developmental processes and associated disorders, such as neurodevelopmental diseases. The production of ASCL3 as a recombinant protein enables detailed biochemical and biophysical analyses, which are crucial for unraveling the complexities of its function and impact on neuronal development. Thus, ongoing studies into the characterization and applications of ASCL3 recombinant protein are vital for advancing our understanding of both basic biology and potential therapeutic strategies.
