Cat: PA2000-1576

Recombinant Human PAP Protein,His

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Analytical Data

  • Gene name

    PAP

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    PAP;PAP;Poly(A) polymerase alpha

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    P15309

  • Expression Region

    33-386aa

  • AA Sequence

    KELKFVTLVF RHGDRSPIDT FPTDPIKESS WPQGFGQLTQ LGMEQHYELG EYIRKRYRKF LNESYKHEQV YIRSTDVDRT LMSAMTNLAA LFPPEGVSIW NPILLWQPIP VHTVPLSEDQ LLYLPFRNCP RFQELESETL KSEEFQKRLH PYKDFIATLG KLSGLHGQDL FGIWSKVYDP LYCESVHNFT LPSWATEDTM TKLRELSELS LLSLYGIHKQ KEKSRLQGGV LVNEILNHMK RATQIPSYKK LIMYSAHDTT VSGLQMALDV YNGLLPPYAS CHLTELYFEK GEYFVEMYYR NETQHEPYPL MLPGCSPSCP LERFAELVGP VIPQDWSTEC MTTNSHQGTE DSTDHHHHHH

  • Molecular Weight

    45 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

PAP (Prostatic Acid Phosphatase) is an enzyme primarily produced in the prostate, with significant implications in prostate cancer research and diagnosis. Historically, PAP has been emphasized for its role as a biomarker in prostate cancer, as elevated levels of the enzyme are often associated with disease progression and metastasis. Researchers have sought to better understand the structure and function of PAP, leading to the development of recombinant forms of the protein that can be used for diagnostic and therapeutic purposes. Recombinant PAP is produced using recombinant DNA technology, which allows for the mass production of the protein with high purity and activity. This advancement has paved the way for innovative approaches in both immunotherapy and vaccine development against prostate cancer. Additionally, studies have shown that PAP has potential as an antigen for cancer vaccines, prompting investigations into its use in therapeutic settings. Overall, the research surrounding PAP and its recombinant forms reflects a broad interest in improving prostate cancer diagnostics and treatment strategies, driving significant advancements in the field of oncology.

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