Analytical Data
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Gene name
PDE8A
- Application
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Alternative Names
5''-cyclic phosphodiesterase 8A; cAMP specific cyclic nucleotide phosphodiesterase 8A ; FLJ16150; High affinity cAMP specific and IBMX insensitive 3'5' cyclic phosphodiesterase 8A ; High affinity cAMP-specific and IBMX-insensitive 3''; HsT19550; PDE 8A; PDE8A; PDE8A_HUMAN; Phosphodiesterase 8A ; Phosphodiesterase8A; Weakly similar to 3'5' cyclic nucleotide phosphodiesterase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60658
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Expression Region
1-829 aa
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AA Sequence
MGCAPSIHISERLVAEDAPSPAAPPLSSGGPRLPQGQKTAALPRTRGAGLLESELRDGSGKKVAVADVQFGPMRFHQDQLQVLLVFTKEDNQCNGFCRACEKAGFKCTVTKEAQAVLACFLDKHHDIIIIDHRNPRQLDAEALCRSIRSSKLSENTVIVGVVRRVDREELSVMPFISAGFTRRYVENPNIMACYNELLQLEFGEVRSQLKLRACNSVFTALENSEDAIEITSEDRFIQYANPAFETTMGYQSGELIGKELGEVPINEKKADLLDTINSCIRIGKEWQGIYYAKKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKAEKISECVQSDTHTDNQTGKHKDRRKGSLDVKAVASRATEVSSQRRHSSMARIHSMTIEAPITKVINIINAAQESSPMPVTEALDRVLEILRTTELYSPQFGAKDDDPHANDLVGGLMSDGLRRLSGNEYVLSTKNTQMVSSNIITPISLDDVPPRIARAMENEEYWDFDIFELEAATHNRPLIYLGLKMFARFGICEFLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE
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Molecular Weight
119.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDE8A, or Phosphodiesterase 8A, is an enzyme belonging to the phosphodiesterase family, which plays a crucial role in regulating intracellular levels of cyclic nucleotides, specifically cyclic AMP (cAMP) and cyclic GMP (cGMP). These cyclic nucleotides are vital signaling molecules involved in various physiological processes, including cell proliferation, differentiation, and apoptosis. Dysregulation of PDE8A has been implicated in several diseases, such as cancer, cardiovascular disorders, and neurodegenerative diseases, making it a promising target for therapeutic intervention. The study of recombinant PDE8A proteins has gained attention as researchers aim to elucidate its structure, function, and regulatory mechanisms. This research often involves cloning the PDE8A gene, expressing it in suitable host cells, and purifying the recombinant protein to characterize its enzymatic activity and interaction with potential inhibitors. Understanding the biochemical properties of PDE8A and its role in cellular signaling pathways could provide insights into the development of novel pharmacological agents that modulate its activity, potentially leading to innovative treatments for various diseases. Consequently, PDE8A not only represents an important focus in basic biological research but also holds significant therapeutic potential in the field of medicine.
