Analytical Data
-
Gene name
TNFA
- Application
-
Alternative Names
TNFA；TNFA；Tumor necrosis factor
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P01375
-
Expression Region
77-233aa
-
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
-
Molecular Weight
19.4 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Tumor Necrosis Factor Alpha (TNFA) is a pivotal cytokine involved in systemic inflammation and is a key regulator of the immune response. Its role extends to various physiological processes, including cell proliferation, differentiation, and apoptosis. TNFA is primarily secreted by macrophages and can influence a wide range of biological activities. Given its critical involvement in inflammation and immune regulation, TNFA has been a focus of research in various fields, including immunology, cancer biology, and autoimmune diseases. Recombinant TNFA proteins have been developed for various therapeutic and research applications. These proteins are used to study TNFA's mechanisms of action, signaling pathways, and its effects on different cell types. Furthermore, the exploration of TNFA's role in diseases like rheumatoid arthritis, Crohn's disease, and cancer has led to the development of targeted therapies, such as TNFA inhibitors. Understanding the structure-function relationship of TNFA and its receptors could provide insights into novel therapeutic strategies and biomarker development. The synthesis and characterization of recombinant TNFA have also advanced with the use of biotechnology, enabling more precise studies in vitro and in vivo. As research progresses, the therapeutic potential of TNFA and its analogs continues to be a promising area, addressing unmet medical needs related to inflammatory disorders and malignancies.
