Analytical Data
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Gene name
ARIH2
- Application
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Alternative Names
HT005; all trans retinoic acid inducible RING finger; Ari 2; Ari-2; ARI2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95376
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Expression Region
1-493aa
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AA Sequence
MSVDMNSQGS DSNEEDYDPN CEEEEEEEED DPGDIEDYYV GVASDVEQQG ADAFDPEEYQ FTCLTYKESE GALNEHMTSL ASVLKVSHSV AKLILVNFHW QVSEILDRYK SNSAQLLVEA RVQPNPSKHV PTSHPPHHCA VCMQFVRKEN LLSLACQHQF CRSCWEQHCS VLVKDGVGVG VSCMAQDCPL RTPEDFVFPL LPNEELREKY RRYLFRDYVE SHYQLQLCPG ADCPMVIRVQ EPRARRVQCN RCNEVFCFKC RQMYHAPTDC ATIRKWLTKC ADDSETANYI SAHTKDCPKC NICIEKNGGC NHMQCSKCKH DFCWMCLGDW KTHGSEYYEC SRYKENPDIV NQSQQAQARE ALKKYLFYFE RWENHNKSLQ LEAQTYQRIH EKIQERVMNN LGTWIDWQYL QNAAKLLAKC RYTLQYTYPY AYYMESGPRK KLFEYQQAQL EAEIENLSWK VERADSYDRG DLENQMHIAE QRRRTLLKDF HDT
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Molecular Weight
57.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARIH2, an E3 ubiquitin ligase, plays a crucial role in regulating protein degradation and cellular homeostasis through the ubiquitin-proteasome system. It is involved in various biological processes, including cell cycle regulation, DNA repair, and response to stress, making it a significant target for understanding cellular mechanisms and disease pathology. Research has shown that ARIH2 is associated with certain cancers and neurodegenerative disorders, highlighting its potential as a biomarker and therapeutic target. Recent studies have focused on characterizing the structure and function of recombinant ARIH2 proteins, aiming to elucidate their interactions with substrate proteins and other regulatory molecules. This work is vital for understanding how ARIH2 modulates cellular processes and could lead to the development of innovative strategies for cancer treatment and other diseases related to protein misfolding and degradation anomalies. By exploring the enzymatic activity and binding partners of ARIH2, researchers hope to develop small molecules or biologics that can modulate its activity and restore normal protein turnover in diseased cells. Overall, the investigation of ARIH2 recombinant protein is positioned at the intersection of basic biology and therapeutic development, offering promising avenues for future research and intervention in various health challenges.
