Analytical Data
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Gene name
ARIH1
- Application
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Alternative Names
ARI 1; ARI; ARI-1; ARI1; ARI1_HUMAN; Ariadne Drosophila homolog of
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y4X5
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Expression Region
1-557aa
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AA Sequence
MDSDEGYNYE FDEDEECSEE DSGAEEEEDE DDDEPDDDTL DLGEVELVEP GLGVGGERDG LLCGETGGGG GSALGPGGGG GGGGGGGGGG PGHEQEEDYR YEVLTAEQIL QHMVECIREV NEVIQNPATI TRILLSHFNW DKEKLMERYF DGNLEKLFAE CHVINPSKKS RTRQMNTRSS AQDMPCQICY LNYPNSYFTG LECGHKFCMQ CWSEYLTTKI MEEGMGQTIS CPAHGCDILV DDNTVMRLIT DSKVKLKYQH LITNSFVECN RLLKWCPAPD CHHVVKVQYP DAKPVRCKCG RQFCFNCGEN WHDPVKCKWL KKWIKKCDDD SETSNWIAAN TKECPKCHVT IEKDGGCNHM VCRNQNCKAE FCWVCLGPWE PHGSAWYNCN RYNEDDAKAA RDAQERSRAA LQRYLFYCNR YMNHMQSLRF EHKLYAQVKQ KMEEMQQHNM SWIEVQFLKK AVDVLCQCRA TLMYTYVFAF YLKKNNQSII FENNQADLEN ATEVLSGYLE RDISQDSLQD IKQKVQDKYR YCESRRRVLL QHVHEGYEKD LWEYIED
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Molecular Weight
64.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The ARIH1 protein, an E3 ubiquitin ligase, plays a pivotal role in cellular processes such as protein degradation, signal transduction, and DNA damage response through its regulation of ubiquitination. Ubiquitination, the process by which ubiquitin molecules are attached to a target protein, can influence a protein's stability, localization, and activity. Research has shown that ARIH1 is crucial for maintaining cellular homeostasis and modulating various biological functions, including immune response and cell division. Its dysregulation has been implicated in several diseases, including cancer and neurodegenerative disorders, highlighting the need to understand its mechanisms and interactions. The recombinant expression of ARIH1 allows for the study of its functional characteristics, substrate specificity, and potential as a therapeutic target. By generating and characterizing ARIH1 recombinant proteins, researchers can delve deeper into its enzymatic activity and role in disease pathology, paving the way for the development of novel interventions that could restore normal cellular functions affected by ARIH1 dysfunction.
