Analytical Data
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基因名
ARAF
- Application
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别名
ARAF；ARAF1；PKS；PKS2；Serine/threonine-Protein kinase A-Raf
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10398
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表达区间
1-606aa
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氨基酸序列
MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS DGTPRGSPSP ASVSSGRKSP HSKSPAEQRE RKSLADDKKK VKNLGYRDSG YYWEVPPSEV QLLKRIGTGS FGTVFRGRWH GDVAVKVLKV SQPTAEQAQA FKNEMQVLRK TRHVNILLFM GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGCRDQIIF MVGRGYLSPD LSKISSNCPK AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS AARLVP
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分子量
67.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ARAF (Araf-related protein) is a member of the RAF kinase family, which plays a pivotal role in the RAS signaling pathway that regulates cellular proliferation, differentiation, and survival. Research into ARAF's structure and function has gained momentum due to its involvement in various cancers and its potential as a therapeutic target. Unlike its more extensively studied relatives, A-RAF and B-RAF, ARAF has been less characterized, leading researchers to investigate its unique regulatory mechanisms and biological significance. Studies have revealed that ARAF can act as both a tumor suppressor and an oncogene, depending on the context and cellular environment. Understanding the mechanisms that govern ARAF's activity is crucial, as it may help in the development of targeted therapies for RAS-driven malignancies. Moreover, advances in recombinant DNA technology have enabled the production of ARAF proteins for in vitro studies, facilitating investigations into its interactions with other signaling molecules and its downstream effects. As the landscape of cancer treatment continues to evolve, elucidating the role of ARAF will not only enhance our understanding of RAF kinases but also contribute to the development of innovative therapeutic strategies aimed at inhibiting aberrant signaling pathways in cancer.
