Analytical Data
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Gene name
HARS2
- Application
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Alternative Names
HARS2；HARSL；HARSR；HO3；Histidine--tRNA ligase. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49590
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Expression Region
34-506aa
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AA Sequence
QSQVAEAVLTSQLKAHQEKPNFIIKTPKGTRDLSPQHMVVREKILDLVISCFKRHGAKGMDTPAFELKETLTEKYGEDSGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKVKKMKRYHVGKVWRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES
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Molecular Weight
69.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HARS2, or Histidyl-tRNA synthetase 2, is an essential enzyme involved in the process of protein synthesis, specifically in the aminoacylation of tRNA with histidine. Mutations in the HARS2 gene have been linked to various diseases, including mitochondrial disorders and other genetic syndromes. Research into HARS2 recombinant proteins has intensified due to its critical role in cellular function and potential implications in understanding the molecular basis of related diseases. The study of HARS2 not only provides insights into the intricate mechanisms of amino acid incorporation during translation but also highlights its significance in mitochondrial health and metabolism. Furthermore, as mitochondrial dysfunction is a common feature in numerous conditions, recombinant HARS2 proteins can serve as valuable tools in elucidating the pathophysiology of these disorders. Investigating the structure, function, and interaction of HARS2 proteins through recombinant techniques can also pave the way for developing novel therapeutic strategies aimed at correcting the underlying issues caused by HARS2 mutations. As such, the exploration of HARS2 recombinant proteins stands at the intersection of fundamental biological research and clinical translation, offering prospects for enhanced understanding and treatment of mitochondrial-related diseases.
